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APPLICATION OF SOLID-STATE FERMENTATION FOR CELLULASE ENZYME PRODUCTION USING TRICHOD
Post: #1

APPLICATION OF SOLID-STATE FERMENTATION FOR CELLULASE ENZYME PRODUCTION USING TRICHODERMA VIRIDE


.pdf  30_V5_MACEDONIA_PIEB_Kiro Mojsov_d.pdf (Size: 72.37 KB / Downloads: 35)


Introduction

For solid-state fermentation (SSF) processes, different
agro-industrial wastes are used as solid substrates. Selection
of agro-industrial residues for utilization in SSF depends on
some physical parameters such as particle size, moisture
level, intra-particle spacing and nutrient composition within
the substrate. In recent years, some important agroindustrial
residues such as sugarcane bagasse, sugar beat
pulp/husk, orange bagasse, oil cakes, apple pomace, grape
juice, grape seed, coffee husk, wheat bran, cereals, straw,
leaves, corncobs etc. have been used as substrates for solidstate
fermentation.


Biomass production measurements

Biomass production was measured as dry weight (mg).
After filtering, the substrate and the retained cell mass was
dried at 70 oC in an oven until constant weight and then
measured. The content of protein in base was estimated
according to method of Keldhal [mg biomass = mg protein
X 2].

Results and discussion

Solid State Fermentation (SSF) is applied for the
processes in which insoluble materials in water are used for
the microbial growth (Moo-Young et al.,1983). Water is
essential for the microbial growth and in SSF and it is
present in thin layers and in occasions, absorbed inside the
substrates (Mudgett, 1986).


Conclusion

Agricultural waste in the form of cellulose which is the
most abundant renewable biomass in the biosphere has been
shown to be used in the production of valuable products by
microorganism. Wheat straw and alkali treatment wheat
straw are some of these agricultural wastes used in this
work as fermentation substrate which produced a large
amount of cellulase enzymes by Trichoderma viride. These
results highlight the industrial potentials of the substrates as
possible raw materials for cellulase enzyme production by
Trichoderma viride.
Post: #2
Solid-state fermentation is an alternative to submerged fermentation to produce antibiotics, monocellular proteins, enzymes, organic acids, biofuels, etc. However, the advantages of SSF in various processes are greater than in submerged fermentation. This technique not only lowers the cost of the process but also makes the product cheaper for consumers. The article describes the experimental application of SSF in wheat straw for the production of mycelial proteins and cellulase enzymes by Trichoderma viride. This actual residue from the agricultural industry was used as a nutritional base by Trichoderma viride in SSF for the production of cellulase enzymes. Growth and enzyme production by Trichoderma viride were evaluated in wheat straw and alkaline treatment wheat straw (wet processing). Growth of the microorganism (biomass content) shows maximum (123.44 mg / petri dish) in comparative alkaline treatment wheat straw (96.36 mg / Petri dish) in wheat straw for 240 hours. The results show that wheat straw residues from the agricultural industry can be used as an inexpensive base (carbon source) for the industrial production of cellulase enzymes by Trichoderma viride.
 

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